Reaction per well (µl)
Number of reaction (well)
Template volume per well (µl)
Buffer (X)
MgCl2 in Buffer (mM in stock)
ComponentsStockFinal
Taq (U/µl)
dNTP (mM each)
MgCl2 (mM)
Primer F (Stok:pmol/µl (µM); Final:nM)
Primer R (Stok:pmol/µl (µM); Final:nM)
Probe (Stok:pmol/µl (µM); Final:nM)

Components
Single tube (µl)
Total (µl)
Water
PCR Buffer
MgCl2
dNTP
Primer F
Primer R
Probe
Taq
Template
Total (Exc. template)
Total (Inc. template)
program by uozyilmaz 2021

Primers/Probe
Loading order
PCR cond.
Suppliers
Gel/Run.
Visual
04/18/2024 04:33:48 am