Reaction per well (µl)
Number of reaction (well)
Template volume per well (µl)
Buffer (X)
MgCl2 in Buffer (mM in stok)
ComponentsStokFinal
Taq (U/µl)
dNTP (mM each)
MgCl2 (mM)
Primer F (Stok:pmol/µl (µM); Final:nM)
Primer R (Stok:pmol/µl (µM); Final:nM)
Probe (Stok:pmol/µl (µM); Final:nM)

Components
Bir tüp (µl)
Toplam (µl)
Water
PCR Buffer
MgCl2
dNTP
Primer F
Primer R
Probe
Taq
Template
Total (Exc. template)
Total (Inc. template)
program by uozyilmaz 2021
08/12/2022 03:04:19 am